Corning® Ham's F-10 Medium
By Corning®
Shipped at 15 to 30°C; store at 2 to 8°C.
Ham's F-10 Medium Formulation
| Catalog No. | 23-10-070 1X, Liquid Ham's F-10 | 23-50-030 Powder Ham's F-10 |
|---|---|---|
| Inorganic Salts | ||
| CaCl2 (anhydrous) | 33.30mg/L | 33.30mg/L |
| CuSO4 (anhydrous) | 0.0016 | 0.0016 |
| FeSO4•7H2O | 0.834 | 0.834 |
| KCl | 285.00 | 285.00 |
| KH2PO4 | 83.00 | 83.00 |
| MgSO4 (anhydrous) | 74.60 | 74.60 |
| NaCl | 7400.00 | 7400.00 |
| Na2HPO4 (anhydrous) | 153.70 | 153.70 |
| NaHCO3 | 1200.00 | - |
| ZnSO4•7H2O | 0.0288 | 0.0288 |
| Amino acids | ||
| L-Alanine | 9.00 | 9.00 |
| L-Arginine•HCl | 211.00 | 211.00 |
| L-Asparagine•H2O | 15.00 | 15.00 |
| L-Aspartic acid | 13.00 | 13.00 |
| L-Cysteine•HCl•H2O | 35.10 | 35.10 |
| L-Glutamic acid | 14.70 | 14.70 |
| L-Glutamine | 146.20 | 146.20 |
| Glycine | 7.50 | 7.50 |
| L-Histidine•HCl•H2O | 23.00 | 23.00 |
| L-Isoleucine | 2.60 | 2.60 |
| L-Leucine | 13.00 | 13.00 |
| L-Lysine•HCl | 29.00 | 29.00 |
| L-Methionine | 4.48 | 4.48 |
| L-Phenylalanine | 5.00 | 5.00 |
| L-Proline | 11.50 | 11.50 |
| L-Serine | 10.50 | 10.50 |
| L-Threonine | 3.57 | 3.57 |
| L-Tryptophan | 0.60 | 0.60 |
| L-Tyrosine•2Na•2H2O | 2.61 | 2.61 |
| L-Valine | 3.50 | 3.50 |
| Vitamins | ||
| Biotin | 0.024 | 0.024 |
| D-Calcium pantothenate | 0.715 | 0.715 |
| Choline chloride | 0.698 | 0.698 |
| Folic acid | 1.32 | 1.32 |
| i-Inositol | 0.541 | 0.541 |
| Nicotinamide | 0.615 | 0.615 |
| Pyridoxine•HCl | 0.206 | 0.206 |
| Riboflavin | 0.376 | 0.376 |
| Thiamine•HCl | 1.00 | 1.00 |
| Vitamin B12 | 1.36 | 1.36 |
| Other | ||
| D-Glucose | 1100.00 | 1100.00 |
| Hypoxanthine, Na | 4.77 | 4.77 |
| Lipoic acid | 0.20 | 0.20 |
| Methyl lineoleate | - | - |
| Phenol red, Na | 1.20 | 1.20 |
| Putrescine•2HCl | - | - |
| Sodium pyruvate | 110.00 | 110.00 |
| Thymidine | 0.73 | 0.73 |
| Add | ||
| NaHCO3 Powder 7.5% Solution | - - | 1.20g/L 16.0mL/L |
| Specifications | ||
| pH (before buffer) | N/A | 6.5±0.5 |
| pH (after buffer) | 7.2±0.2 | 7.5±0.5 |
| Osmolality (mOsm) | 290±30 | 290±30 |
| Grams of powder required to prepare 1L (1X Solution) | N/A | 9.82 |