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Corning® cellgro® Basal Salt Solutions

By Corning® cellgro®

Delbecco's Phosphate-Buffered Saline (DPBS) and Phosphate Buffered Salts (PBS).

Physio-Chemical Properties

Basal salt solutions undergo testing to determine pH and osmolality according to specific protocols which are traceable to the National Institute of Standards and Technology.

Biological Tests

Mediatech prides itself on its ability to guarantee low levels of endotoxin, the absence of mycoplasma, and a random contamination rate so low that it allows Mediatech products to be labeled "sterile."

Endotoxin: Media are tested for bacterial endotoxin using the Limulus Amoebocyte Lysate (LAL) Chromogenic Assay, following guidelines outlined by the manufacturer and the FDA (1987). For release, each lot of standard liquid media must have an endotoxin level of <0.25 EU/mL. The majority of Mediatech's liquid media are at or below the lowest detectable level today (<0.005 EU/mL). Powder products must have an endotoxin level of <0.25 EU/mL when reconstituted to formula weight with low-endotoxin water.

Sterility: Mediatech selects a representative number of samples from each production lot to test using the membrane filtration technique for 14 days, in accordance with USP XXIV Chapters 71 and 1211.

Mycoplasma: Media products are tested for mycoplasma offsite using a large-volume method described by Kern and Barile in "Isolation of Mycoplasmas from Cell Culture by Agar and Broth Techniques," M.F. Barile and G.J. McGarrity (1983) in Methods of Mycoplasmology, Vol. 11,5. Razin and J.G. Tulley, eds., New York: Academic Press, 159-165.

Dulbecco's Phosphate-Buffered Saline (DPBS) & Phosphate-Buffered Saline (PBS) composition

Cat. No. 23-20-030 10X, Liquid DPBS 23-20-031 10X, Liquid DPBS 23-21-030 1X, Liquid DPBS 23-21-031 1X, Liquid DPBS 23-55-031 Powder DPBS 23-21-040 1X, Liquid PBS
Inorganic Salts
CaCl2 (anhydrous) 1.00g/L - 0.10g/L - - -
KCl 2.00 2.00g/L 0.20 0.20g/L 0.20g/L -
KH2PO4 2.00 2.00 0.20 0.20 0.20 0.144
MgCl2•6H2O 1.00 - 0.10 - - -
NaCl 80.00 80.00 8.00 8.00 8.00 9.00
Na2HPO4 (anhydrous) 11.50 11.50 - 1.15 1.15 0.795
Na2HPO4•7H2O - - 2.1716 - - -
Specifications
pH (before adjustment) N/A N/A N/A N/A 7.4±0.1 N/A
pH (final) 4.4±0.3 6.5±0.5 7.0±0.1 7.4±0.1 N/A 7.4±0.1
Osmolality (mOsm) 276±30 @ 1:10 276±30 @ 1:10 276±12 276±10 282±15 287±15
Grams of powder required to prepare 1L (1X Solution) N/A N/A N/A N/A 9.55 N/A
Cat.No. Description Pack Size Shipped Storage Intended Use
Dulbecco's Phosphate-Buffered Saline (DPBS)
23-20-030-CV Dulbecco's Phosphate-Buffered Saline, 10X with calcium & magnesium 6 x 500 mL A R/A I
23-20-031-CV Dulbecco's Phosphate-Buffered Saline, 10X without calcium & magnesium 6 x 500 mL A R/A I
23-21-030-CV Dulbecco's Phosphate-Buffered Saline, 1X with calcium & magnesium 6 x 500 mL A R/A I
23-21-030-CM Dulbecco's Phosphate-Buffered Saline, 1X with calcium & magnesium 6 x 1 L A R/A I
23-21-031-CV Dulbecco's Phosphate-Buffered Saline, 1X without calcium & magnesium 6 x 500 mL A R/A I
23-21-031-CM Dulbecco's Phosphate-Buffered Saline, 1X without calcium & magnesium 6 x 1 L A R/A I
23-21-031-LX** Dulbecco's Phosphate-Buffered Saline, 1X without calcium & magnesium 1 x 10 L A R/A I
23-21-031-LB** Dulbecco's Phosphate-Buffered Saline, 1X without calcium & magnesium 1 x 20 L A R/A I
23-55-031-PB Dulbecco's Phosphate-Buffered Saline, Powder without calcium & magnesium 1 x 10 L A R IVD
23-55-031-PC Dulbecco's Phosphate-Buffered Saline, Powder without calcium & magnesium 1 x 50 L A R IVD
Phosphate-Buffered Saline (PBS)
23-21-040-CV Phosphate-Buffered Saline, 1X without calcium & magnesium 6 x 500 mL A R/A I
23-21-040-CM Phosphate-Buffered Saline, 1X without calcium & magnesium 6 x 1 L A R/A I

F = -5 to -20°C
R = 2 to 8°C
A = 15 to 30°C
* = Glass
** = Flexible Media Bag
I = Sterile, IVD Product
NI = Sterile, Research Use Only, Not for IVD Use
IVD = In Vitro Diagnostic
Res = Research Use Only, Not for IVD Use

                 
Cat.No. Name Addl. Info. EA   Certified PK    
23-21-030-CV DPBS, 1X w/ calcium, magnesium 6-pack / 500mL bottles       $86.87
1/PK
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23-21-030-CM DPBS, 1X w/ calcium, magnesium 6-pack / 1L bottles       $133.51
1/PK
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23-20-030-CV DPBS, 10X w/ calcium, magnesium 6-pack / 500mL bottles       $155.66
1/PK
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23-21-031-CV DPBS, 1X w/o calcium, magnesium 6-pack / 500mL bottles       $79.73
1/PK
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23-21-031-CM DPBS, 1X w/o calcium, magnesium 6-pack / 1L bottles       $132.18
1/PK
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23-20-031-CV DPBS, 10X w/o calcium, magnesium 6-pack / 500mL bottles       $134.28
1/PK
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23-21-040-CV PBS 1X w/o calcium, magnesium 6-pack / 500mL bottles       $71.41
1/PK
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23-21-040-CM PBS 1X w/o calcium, magnesium 6-pack / 1L bottles       $127.50
1/PK
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23-21-031-LB DPBS, 1X w/o calcium, magnesium 20L $356.04
1/EA
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23-21-031-LX DPBS, 1X w/o calcium, magnesium 10L $214.64
1/EA
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23-55-031-PB DPBS, powder, w/o calcium, magnesium 10L $24.35
1/EA
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23-55-031-PC DPBS, powder, w/o calcium, magnesium 50L $37.14
1/EA
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