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Corning® BioCoat™ Smooth Muscle Cell Differentiation Environment

By CorningĀ® BioCoatĀ®
Corning® BioCoat™ Smooth Muscle Cell Differentiation Environment

An in vitro system optimized for the differentiation of human and rat SMC. Includes specially formulated Corning BioCoat™ Growth Factor Reduced (GFR) Matrigel™ Matrix Cellware and serum-free Smooth Muscle Differentation Medium.

  • Three-dimensional matrix promotes differentiated (contractile) SMC phenotype, low proliferative index, nodular morphology and ultrastructure dominated by microtubules and microfilaments
  • Expression of cytoskeletal markers, α-smooth muscle actin, heavy chain myosin, and 150 kD h-caldesmon
  • Carry out studies of SMC specific function two weeks earlier than Collagen I
  • Reduced levels of growth factors for cytokine studies
  • Serum-free environment maximizes control over experimental conditions

Complete protocol provides:

  • Standardized procedures
  • Capability for immediate start-up

Applications:

Corning Matrigel™ Matrix has been used to culture SMC for studies of:

  • SMC differentiation
  • Modulation of SMC function
  • Molecular mechanisms of phenotypic transitions
  • Effects of various compounds on SMC behavior
  • Effects of ECM proteins on vascular SMC phenotype
 
                                 
Cat.No.BrandNameTypeTreatedNumber of WellsMaterialWell ShapeColorLidGrowth AreaSterilePackagingAddl. Info.CS  
3-354635 BioCoat™   Smooth muscle cell differentiation environment Y 24 Polystyrene Flat Clear Y 2.0cm² N Pack of 5 Matrigel™ coated $363.03
5/CS
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