Corning® BioCoat™ Matrigel™ Invasion Chambers
By Corning® BioCoat®
An in vitro system for the study of cell invasion through basement membrane. Consists of Corning Falcon® Cell Culture Inserts containing an 8µm pore-size PET membrane coated with a uniform layer of Corning Matrigel™ Basement Membrane Matrix.
- Discriminating, reproducible, ready-to-use
- Invasivity of HT-1080 cells is at least fivefold greater than 3T3 cells
- Uniform layer of Matrigel™ Matrix occluding the 8µm pores of PET microporous membrane
- An authentic model of in vivo basement membrane
- Barrier to noninvasive cells
- Proprietary coating process
- Only invasive cells digest the matrix and move through the insert membrane
Complete protocol provides:
- Standardized procedures
- Capability for immediate start-up
- Instructions for quantitating percent invasion
Applications:
Corning BioCoat™ Matrigel Invasion Chambers enable studies of:
- Metastatic potential of tumor cells
- Expression of matrix metalloproteinase on surface of invasive tumor cells
- Inhibition of metastasis by ECM components or antineoplastic drugs (i.e., taxol)
- Altered expression of cell surface proteins in metastatic cells
- Invasion of normal cells, such as embryonic stem cells cytotrophoblasts and fibroblasts
- Effects of doxycycline on invasion and metalloproteinase activity of breast carcinoma cells
- Metastatic potential of a newly isolated bladder cancer cell line
- Suppression of lung metastasis of mouse melanoma by gene transfection