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Corning® BioCoat™ Matrigel™ Invasion Chambers

By Corning® BioCoat®
Corning® BioCoat™ Matrigel™ Invasion Chambers

An in vitro system for the study of cell invasion through basement membrane. Consists of Corning Falcon® Cell Culture Inserts containing an 8µm pore-size PET membrane coated with a uniform layer of Corning Matrigel™ Basement Membrane Matrix.

  • Discriminating, reproducible, ready-to-use
  • Invasivity of HT-1080 cells is at least fivefold greater than 3T3 cells
  • Uniform layer of Matrigel™ Matrix occluding the 8µm pores of PET microporous membrane
  • An authentic model of in vivo basement membrane
  • Barrier to noninvasive cells
  • Proprietary coating process
  • Only invasive cells digest the matrix and move through the insert membrane

Complete protocol provides:

  • Standardized procedures
  • Capability for immediate start-up
  • Instructions for quantitating percent invasion

Applications:

Corning BioCoat™ Matrigel Invasion Chambers enable studies of:

  • Metastatic potential of tumor cells
  • Expression of matrix metalloproteinase on surface of invasive tumor cells
  • Inhibition of metastasis by ECM components or antineoplastic drugs (i.e., taxol)
  • Altered expression of cell surface proteins in metastatic cells
  • Invasion of normal cells, such as embryonic stem cells cytotrophoblasts and fibroblasts
  • Effects of doxycycline on invasion and metalloproteinase activity of breast carcinoma cells
  • Metastatic potential of a newly isolated bladder cancer cell line
  • Suppression of lung metastasis of mouse melanoma by gene transfection
 
                           
Cat.No.BrandTypeTreatedNumber of WellsMaterialColorLidGrowth AreaPackagingAddl. Info.CS  
3-354480 BioCoat™ Matrigel™ invasion chambers, two 24-well plates Y 24 PET Clear Y 0.3cm² Pack of 24 PET membrane with 8.0µm pore size, Matrigel™ coated $338.26
24/CS
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3-354481 BioCoat™ Matrigel™ invasion chambers, four 6-well plates Y 6 PET Clear Y 4.2cm² Pack of 24 PET membrane with 8.0µm pore size, Matrigel™ coated $372.57
24/CS
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3-354483 BioCoat™ Growth factor reduced Matrigel™ invasion chambers, two 24-well plates Y 24 PET Clear Y 0.3cm² Pack of 24 PET membrane with 8.0µm pore size, Matrigel™ coated $344.38
24/CS
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