Corning BioCoat™ Tumor Invasion Systems are ideal for use in secondary cell-based screening assays of prospective anti-metastatic compounds. This proprietary system is suitable for both manual and robotic uses and is compatible with most fluorescence plate readers, robots, and fluid handling instruments.
This in vitro system for the study of tumor cell invasion through basement membrane consists of a Corning Falcon® FluoroBlok™ 24-multiwell insert plate containing an 8.0µm pore size FluoroBlok™ membrane coated with a uniform layer of Matrigel Basement Membrane Matrix. The Matrigel Matrix occludes the pores of the FluoroBlok™ membrane, blocking non-invasive cells from migrating through the membrane. Patented membrane is made of light-tight polyethylene terephathalate (PET), efficiently blocking the transmission of light within the range of 490-700nm, allowing fluorescence detection in a simplified and non-destructive manner.
Each lot of BioCoat™ Tumor Invasion System is tested for its ability to allow invasion of HT-1080 cells, an invasive human fibrosarcoma cell line, and to exclude invasion of NIH-3T3 cells, a non-invasive fibroblast cell line. All lots are tested and found negative for bacteria and fungi.