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Anti-Human Lambda Light Chain antibody, Rabbit monoclonal

SIGMA/SAB5600220 - recombinant, expressed in HEK 293 cells, clone RM127, purified immunoglobulin

Synonym: Human Immunoglobulin lambda light chain; IGLC; Ig Lambda Chain C region; Immunoglobulin lambda constant 1 antibody

Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-SAB5600220-100UG 100 µg
$477.00
1/EA
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45-SAB5600220-1MG 1 mg
$3190.00
1/EA
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Immunohistochemistry Immunohistochemistry of Human Tonsil using Anti-Lambda Light Chain antibody RM127.
ELISA ELISA of Human immunoglobulins shows RM127 reacts to the lambda light chain of Human immunoglobulins. No cross reactivity with the kappa light chain, Mouse IgG, Rat IgG, or Goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL or 50 ng/mL of RM127 was used as the primary antibody. An alkaline phosphatase conjugated Anti-Rabbit IgG as the secondary antibody.
ELISA A titer ELISA using RM127. The plate was coated with different amounts of Human IgG3l. A serial dilution of RM127 was used as the primary antibody. An alkaline phosphatase conjugated Anti-Rabbit IgG as the secondary antibody.

 

antibody form purified immunoglobulin
antibody product type primary antibodies
clone recombinant monoclonal
  RM127, monoclonal
form buffered aqueous glycerol solution
isotype IgG
NCBI accession no. 3537 
recombinant expressed in HEK 293 cells
shipped in wet ice
species reactivity human
storage temp. −20°C
technique(s) ELISA: 0.05-0.2 μg/mL
  flow cytometry: suitable (assay dependent concentration)
  immunocytochemistry: 0.5-2 μg/mL
  immunohistochemistry: 0.5—2 μg/mL
UniProt accession no. P0CG04 
Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Immunogen: Human IgG
Physical form: Solution in phosphate buffered saline containing 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide.
Specificity: This antibody reacts to the lambda light chain of human immunoglobulins, and does not react to the kappa light chain. RM127 does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG.
WGK Germany WGK 2
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Storage Temp. −20°C
UNSPSC 12352203

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