L-Glutamine
SIGMA/G6392 - γ-irradiated, BioXtra, suitable for cell culture
Synonym: (S)
CAS Number: 56-85-9
Empirical Formula (Hill Notation): C5H10N2O3
Molecular Weight: 146.14
EC Number: 200-292-1
MDL Number: MFCD00008044
Linear Formula: H2NCOCH2CH2CH(NH2)CO2H
Product Type: Chemical
color | white to faint yellow |
form | powder |
impurities | endotoxin, tested |
InChI | 1S/C5H10N2O3/c6-3(5(9)10) |
InChI key | ZDXPYRJPNDTMRX-VKHMYHEASA |
mp | 185 °C (dec.) (lit.) |
packaging | vial of 0.292 g |
product line | BioXtra |
Quality Level | 200 |
SMILES string | N[C@@H](CCC(N)=O)C(O)=O |
solubility | H2O: 10 mL/vial, clear to slightly hazy |
sterility | γ-irradiated |
technique(s) | cell culture | mammalian: suitable |
Application: | L-Glutamine has been used as a media supplement: • for cochlear organotypic culturing • in Iscove′s modified Dulbecco′s medium for HeLa cells culturing • in Dulbecco′s modified Eagle′s medium for primary hepatocyte cell culturing |
Biochem/physiol Actions: | |
Biochem/physiol Actions: | Glutamine mediates protein turnover via cellular mTOR (mammalian target of rapamycin) signalling. It is also known to be associated with the inhibition of apoptosis. |
Biochem/physiol Actions: | L-glutamine is an essential amino acid that is a crucial component of culture media, and serves as a major energy source for cells in culture. Optimal cell performance usually requires supplementation of the medium with L-glutamine prior to use. |
Other Notes: | The dipeptide L-Ala-LGln is frequently used in modern serum-free media formulations because it provides a more stable form of glutamine in vitro. |
Packaging: | 50 mL amber serum vial with rubber stopper. |
Reconstitution: | Prepares a 200 mM solution when reconstituted to 10 mL with sterile water. |
Suitability: | Qualified for use in cell culture applications, especially serum-free cell culture. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
mp | 185 °C (dec.) (lit.) |
UNSPSC | 12352209 |