Acylase I, immobilized on Eupergit® C from Aspergillus sp.
SIGMA/01824 - ≥50 U/g moist material
Synonym: Aminoacylase, immobilized; Plexazym® AC
MDL Number: MFCD00081285
Product Type: Chemical
| form | beads |
| Quality Level | 100  |
| specific activity | ≥50 U/g moist material |
| storage temp. | 2-8°C |
| Analysis Note: | moist pearls (dried substance ~30%, pearl diameter 50-100 μm), covalent fixation of the acylase |
| General description: | the immobilized acylase catalyzes the hydrolysis of N-acetyl-DL-amino acid to L-amino acid, the D-form is not attacked |
| Legal Information: | Eupergit is a registered trademark of Röhm GmbH & Co. KG |
| Legal Information: | Plexazym is a registered trademark of Röhm GmbH & Co. KG |
| Other Notes: | The immobilized acylase is used for the convenient resolution of amino acids via the selective deacetylation of N-acetyl-L-amino acids in DL-racemates |
| Reconstitution: | Standard procedure: a 10-20% substrate solution, pH 6-8, with an addition of CoCl2 (10-4 moles) at 33°C was used. Prior to use the polymer was washed with water (50 times bed volumes); when used in a fixed bed reactor, a velocity of flow of 3 bed volumes/h leads to a hydrolysis degree of 80% |
| Unit Definition: | 1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol N-acetyl-L-methionine per minute at pH 8.0 and 25°C |
| RIDADR | NONH for all modes of transport |
| WGK Germany | 3 |
| activity | specific activity: ≥50 U/g moist material |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.5.1.14 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |