Stabilize PCR products prior to sequencing
The SeqDirect™ PCR Cleaning Kit is designed to purify PCR products for fluorescent cycle sequencing using a 20 minute, two-temperature incubation that is amenable to high-throughput processing. This "cleaning" of PCR products is achieved using a proprietary combination of enzymatic and non-enzymatic methods to remove/neutralize PCR primers, dNTPs, starting materials and PCR buffer components. No columns or precipitations are required, so sample loss is nonexistent. Templates applicable to the procedure include single-band and multiple-band PCR products if using specific nested primers. The SeqDirect PCR Cleaning Kit is offered in several sizes as a concentrate to be reconstituted with the provided buffer before use.
Kit Components: SeqDirect Mixx, Buffer Mixx