close

Register

You are about to set up an account. For this process, you will be asked to provide personal information about yourself and possibly your organization. We invite you to read our Privacy Policy concerning any use of this information that you provide to us.

Benefits of setting up an Account:
  • Allows personalized content such as Favorite Items to be viewed.
  • Conveniently stores information to be used at checkout, such as billing and shipping addresses (Payment information is never stored on the website).
  • Get customized pricing for your organization (when available).

Join Our Mailing List!

Preferred Email Format

* Required

 
Advanced Search

Terminal Deoxynucleotidyl Transferase

By MP Biomedicals

From bovine thymus.

  • 3'-end labeling of DNA
  • Template independent
  • Production of synthetic homo- and heteropolymers
  • addition of homopolymer tails to vectors and cDNA

Terminal deoxynucleotidyl transferase (TdT) is a primer-dependent DNA polymerase that catalyzes the repeated addition of mononucleotide units from a deoxynucleotide triphosphate to the terminal 3' hydroxyl of single or double-stranded DNA. The reaction is affected by the type of bases added (dATP, dCTP, dGTP and dTTP), the type of divalent cations in the buffer (Mn2+, Co2+, Mg2+), and the terminal structure of DNA (3'-protruding -OH end, blunt end, 3'-recessed -OH end). Thus, it is necessary to find the optimum conditions for each reaction. This polymerization releases inorganic pyrophosphate . TdT is widely used in tailing reactions (cDNA and DNA vector tailing). This enzyme is also used for labeling 3' OH ends of DNA fragments.

Storage: KPO4 100 mM pH 7.2, DTT 1 mM, Glycerol 50%. Store at -20°C
Unit Definition: One unit of TdT catalyzes the addition of one nmol deoxyadenylate in one hour onto a p(dT)6 oligodeoxyribonucleotide at 37°C
Incubation buffer 1x: Cacodylate K 140 mM pH 7.2, CoCl2 1 mM, ZnSO4 0.33 mM, BSA 10 mg/ml.
Purity: Free from detectable nuclease activity.

Note:
Under the conditions described above, 70-120 dA or dT residues, or 15-30 dC or dG residues per 3'-OH end of DNA can be added.

Concentration: 15 U/µl
Supplied with 5x incubation buffer

           
Cat.No. Name Size EA    
17-EMTDT400 Terminal Deoxynucleotidyl Transferase 400 U $157.27
1/EA
Add To Favorites

The following items have been added to your cart:

Choose a favorite list for this item:

Catalog Number Description Price
$

Request Literature

Please fill out the form below if you want to request literature from Krackeler Scientific.

 

Quick Order

* Required


New Year Price Updates

We are currently working diligently to update our website pricing information for the New Year. If you place an order, you will be acknowledged with any corrected pricing. If you'd like the most current information sooner, please don't hesitate to drop us an email or give us a call and we'd be happy to assist. Thank you for your patience while we are updating.

800-334-7725
office@krackeler.com


Play Video

To Request a Quote

  1. Search or Browse for items and add to them to your Shopping Cart.
  2. Click the "Request Quote" button at the bottom of the Shopping Cart page.
  3. Fill out required fields.
  4. Optionally you can convert to standard checkout mode by choosing a payment type.
  5. Click "Request Quote" at the bottom of the page.

You will be contacted with a quote.

To Order From a Quote

  1. Enter a quote number below
  2. Click "Checkout" (a special place holder item will be added to your cart)
  3. Enter any additional instructions in the comments section at checkout.

Customer service will contact you with an order confirmation.

Back to Top