Muramidase, Mucopeptide glycolhydrolase; EC 220.127.116.11; MW: 14600
This enzyme purified from chicken egg white is provided as an albumin-free freeze dried powder in the chloride form. The enzyme catalyzes the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Its major application is to disrupt the cell walls of certain bacteria, but it may also be used as an antimicrobial agent.
|Storage:||lyophilized white powder at 4°C. Solutions of lysozyme should be stored at -20°C in aliquots.|
|Unit definition:||1 unit is defined as the amount of enzyme that will catalyze a decrease in absorption at 450 nm of 0.001 per minute at pH 7.0 and 25°C (Micrococcus luteus as substrate).|
|Quality control:||DNase not detectable|
|Use:||Lysozyme is suitable (2 mg/mL) for bacterial mucopolysaccharide hydrolysis. For immediate use, lysozyme can be diluted in water. Stock solutions may be prepared in a buffer containing 10 mM Tris (pH 8.0), 1 mM EDTA.|