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By MP Biomedicals

Molecular biology grade

X-Gal (5-Bromo-4-Chloro-3-Indolyl-β-D-galactopyranoside) is a β-galactosidase chromogenic substrate(1,2). In the presence of β-galactosidase, X-Gal is hydrolyzed and yields a blue precipitate. X-Gal is used for the identification and selection of β-galactosidase producing bacteria (bacteria Lac+), which produce blue colonies on agar medium. X-Gal is used at a 40 µg/ml concentration. It is soluble in dimethylformamide.

CAS No: 7240-90-6
MW: 408.64 /mol
EC No: 230-640-8
Formula: C14H15BrClNO6


Assay (HPLC): > 99.0 %
Appearance: White powder
Specific rotation: [α]20D: -62 ± 2
Water content (KF): < 0.2 %
Solubility (2% in DMF): clear solution
RNase, DNase, Protease free

Storage: Store at -20°C

Cat.No. Name Size EA    
17-XGAL0100 X-Gal, mol-bio grade 100mg DISCONTINUED  
17-XGAL0500 X-Gal, mol-bio grade 5 x 100mg DISCONTINUED  
17-XGAL1000 X-Gal, mol-bio grade 1g DISCONTINUED  
17-XGAL5000 X-Gal, mol-bio grade 5 x 1g DISCONTINUED  

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