For 10-70mL samples, Vivacell 70 combines the ease of use of centrifugal devices with the flexibility and control provided by pressurized ultrafiltration cells. It is inexpensive, quick and easy to assemble, requires no tubing connections or stirring mechanisms and can be adapted to equipment availability or to specific user preferences.
For convenience, simply spin in a large capacity centrifuge (rotors accepting 250mL bottles). For highest speeds particularly with difficult samples, pressurize the device with air or inert gas before centrifuging. For more process control or for single samples, combine gas pressure with a gentle orbital shake, or even pressurize and then leave standing on a bench top or in a refrigerator for highest simplicity with minimum equipment requirements.
The longitudinal membrane inhibits fouling, while the built-in dead stop will hinder further concentration when residual volume drops below 150µL.
BSA 1.0 mg/mL (66,000 MW)
IgG 0.25 mg/mL (160,000 MW)