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Minimum Essential Medium (MEM)

By Corning® cellgro®

Medium is shipped at 15 to 30°C; store at 2 to 8°C.

Minimum Essential Medium Formulation

Catalog No. 23-10-010
1X, Liquid
EMEM
23-15-010
1X, Liquid
EMEM
23-15-015
1X, Liquid
SMEM
23-17-303
1X, Liquid
EMEM
23-17-305
1X, Liquid
EMEM
Inorganic Salts
CaCl2 (anhydrous) 200.00mg/L 200.00mg/L - 200.00mg/L 200.00mg/L
KCl 400.00 400.00 400.00mg/L 400.00 400.00
MgSO4 (anhydrous) 97.70 97.70 - 97.70 97.70
NaCl 6800.00 6800.00 6800.00 6800.00 6800.00
NaH2PO4•H2O 140.00 140.00 1400.00 140.00 140.00
Na2HPO4 - - - - -
NaHCO3 2200.00 2200.00 2200.00 2200.00 2200.00
Amino acids
L-Arginine•HCl 126.40mg/L 126.40mg/L 126.40mg/L 126.40mg/L 126.40mg/L
L-Cystine•2HCl 31.20 31.20 31.20 31.20 31.20
L-Glutamine 292.00 - - - -
L-Histidine•HCl•H2O 41.90 41.90 41.90 41.90 41.90
L-Isoleucine 52.50 52.50 52.50 52.50 52.50
L-Leucine 52.50 52.50 52.50 52.50 52.50
L-Lysine•HCl 72.50 72.50 72.50 72.50 72.50
L-Methionine 15.00 15.00 15.00 - 15.00
L-Phenylalanine 32.50 32.50 32.50 32.50 32.50
L-Threonine 47.60 47.60 47.60 47.60 47.60
L-Tryptophan 10.00 10.00 10.00 10.00 10.00
L-Tyrosine•2Na•2H2O 51.90 51.90 51.90 51.90 51.90
L-Valine 46.80 46.80 46.80 46.80 46.80
Vitamins
D-Calcium pantothenate 1.00mg/L 1.00mg/L 1.00mg/L 1.00mg/L 1.00mg/L
Choline chloride 1.00 1.00 1.00 1.00 1.00
Folic acid 1.00 1.00 1.00 1.00 1.00
i-Inositol 2.00 2.00 2.00 2.00 2.00
Nicotinamide 1.00 1.00 1.00 1.00 1.00
Pyridoxine•HCl 1.00 1.00 1.00 1.00 1.00
Riboflavin 0.10 0.10 0.10 0.10 0.10
Thiamine•HCl 1.00 1.00 1.00 1.00 1.00
Other
D-Glucose 1000.00mg/L 1000.00mg/L 1000.00mg/L 1000.00mg/L 1000.00mg/L
Phenol red, Na 10.00 10.00 10.00 10.00 -
Add
NaHCO3
Powder (g/L)
7.5% Solution (mL/L)

-
-

-
-

-
-

-
-

-
-
L-Glutamine Powder
200 mM Solution (mL/L)

-
-

292.00mg/L
10.00mL/L

292.00mg/L
10.00mL/L

292.00mg/L
10.00mL/L

292.00mg/L
10.00mL/L
Specifications
pH (before buffer) N/A N/A N/A N/A N/A
pH (after buffer) 7.2±0.2 7.2±0.2 7.2±0.2 7.2±0.2 7.2±0.2
Osmolality (mOsm) 290±30 290±30 290±30 290±30 290±30
Grams of powder required to prepare 1L (1X Solution) N/A N/A N/A N/A N/A

Minimum Essential Medium Formulation

Catalog No. 23-50-004
Powder
GMEM
23-50-010
Powder
EMEM
23-50-011
Powder
EMEM
23-50-019
Powder
HMEM
23-51-010
Powder
Autoclavable
EMEM
23-90-009
Powder
EMEM
Inorganic Salts
CaCl2 (anhydrous) 200.00mg/L 200.00mg/L 200.00mg/L 140.00mg/L 200.00mg/L 200.00mg/L
Fe(NO3)3•9H2O 0.10 - - - - -
KCl 400.00 400.00 400.00 400.00 400.00 400.00
KH2PO4 - - - 60.00 - -
MgSO4 (anhydrous) 97.67 97.70 97.70 97.67 97.00 97.70
NaCl 6400.00 6800.00 6800.00 8000.00 6800.00 6800.00
NaH2PO4•H2O 124.00 140.00 140.00 - 140.00 140.00
Na2HPO4 (anhydrous) - - - 47.88 - -
NaHCO3 - - - - - -
Na succinate•6H2O - - - - 100.00 -
Amino acids
L-Alanine - - 8.90mg/L - - -
L-Arginine•HCl 42.00mg/L 126.40mg/L 126.40 126.00mg/L 126.40mg/L 126.40mg/L
L-Asparagine•H2O - - 15.00 - - -
L-Aspartic acid - - 13.30 - - -
L-Cystine•2HCl 31.28 31.20 31.20 31.29 31.10 31.20
L-Glutamic acid - - 14.70 - - -
L-Glutamine 292.00 292.00 292.00 292.00 - -
Glycine - - 7.50 - - -
L-Histidine•HCl•H2O 21.00 41.90 41.90 42.00 41.90 41.90
L-Isoleucine 52.40 52.50 52.50 52.00 52.50 52.50
L-Leucine 52.40 52.50 52.50 52.00 52.50 52.50
L-Lysine•HCl 73.10 72.50 72.50 72.50 73.06 72.50
L-Methionine 15.00 15.00 15.00 15.00 14.90 15.00
L-Phenylalanine 33.00 32.50 32.50 32.00 33.02 32.50
L-Proline - - 11.50 - - -
L-Serine - - 10.50 - - -
L-Threonine 47.60 47.60 47.60 48.00 47.64 47.60
L-Tryptophan 8.00 10.00 10.00 10.00 10.20 10.00
L-Tyrosine•2Na•2H2O 52.19 51.90 51.90 51.90 - 51.90
L-Tyrosine, free base - - - - 36.00 -
L-Valine 46.80 46.80 46.80 46.00 46.90 46.80
Vitamins
Choline bitartrate - - - - 1.80mg/L -
D-Calcium pantothenate 2.00mg/L 1.00mg/L 1.00mg/L 1.00mg/L 1.00 1.00mg/L
Choline chloride 2.00 1.00 1.00 1.00 - 1.00
Folic acid 2.00 1.00 1.00 1.00 1.00 1.00
i-Inositol 3.60 2.00 2.00 2.00 2.00 2.00
Nicotinamide 2.00 1.00 1.00 1.00 1.00 1.00
Pyridoxine•HCl 2.00 1.00 1.00 1.00 1.00 1.00
Riboflavin 0.20 0.10 0.10 0.10 0.10 0.10
Thiamine•HCl 2.00 1.00 1.00 1.00 1.00 1.00
Other
D-Glucose 4500.00mg/L 1000.00mg/L 1000.00mg/L 1000.00mg/L 1000.00mg/L 1000.00mg/L
Phenol red, Na 15.00 10.00 10.00 10.00 6.00 -
Sodium pyruvate - - - - - -
Succinic acid - - - - 75.00 -
Add
NaHCO3
Powder
7.5% Solution

2.75g/L
36.67mL/L

2.20g/L
29.33mL/L

2.20g/L
29.33mL/L

2.20g/L
29.33mL/L

2.20g/L
29.33mL/L

2.20g/L
29.33mL/L
L-Glutamine
Powder
200 mM Solution

-
-

-
-

-
-

-
-

292.00mg/L
10.00mL/L

292.00mg/L
10.00mL/L
Specifications
pH (before buffer) 6.0±0.5 6.0±0.5 5.5±0.5 6.7±0.2 INFO 6.0±0.5
pH (after buffer) 7.5±0.5 7.5±0.5 7.5±0.5 7.2±0.2 INFO 7.5±0.5
Osmolality (mOsm) 290±30 290±30 290±30 285±15 INFO 290±30
Grams of powder required to prepare 1L (1X Solution) 12.52 9.53 9.61 10.63 9.39 9.23
                   
Cat.No. Name Comments Pkg Size EA     PK    
23-10-010-CM MEM with Earle's Salts and L-glutamine   6-pack/1L bottles       $155.17
1/PK
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23-10-010-CV MEM with Earle's Salts and L-glutamine   6-pack/500mL bottles       $99.66
1/PK
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23-15-010-CM MEM with Earle's Salts w/o L-glutamine   6-pack/1L bottles       $181.46
1/PK
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23-15-010-CV MEM with Earle's Salts w/o L-glutamine   6-pack/500mL bottles       $92.35
1/PK
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23-15-015-CV MEM w/o L-glutamine, calcium, magnesium for suspension cultures 6-pack/500mL bottles       $201.98
1/PK
Add To Favorites
23-17-305-CV MEM w/ Earle's salts, w/o L-glutamine, phenol red   6-pack/500mL bottles       $123.57
1/PK
Add To Favorites
23-50-010-PB MEM w/ Earle's salts, L-glutamine, w/o sodium bicarbonate powder 10L bottle $33.71
1/EA
Add To Favorites      
23-50-010-PC MEM w/ Earle's salts, L-glutamine, w/o sodium bicarbonate powder 50L bottle $91.23
1/EA
Add To Favorites      
23-50-011-PB MEM w/ Earle's salts, L-glutamine, nonessential amino acids, w/o sodium bicarbonate powder 10L bottle $32.07
1/EA
Add To Favorites      
23-50-011-PC MEM w/ Earle's salts, L-glutamine, nonessential amino acids, w/o sodium bicarbonate powder 50L bottle $49.71
1/EA
Add To Favorites      
23-50-019-PB MEM w/ Hank's salts, L-glutamine, w/o sodium bicarbonate powder 10L powder No Price        
23-50-019-PC MEM w/ Hank's salts, L-glutamine, w/o sodium bicarbonate powder 50L powder No Price        
23-51-010-PB MEM w/ Earle's salts, w/o L-glutamine, sodium bicarbonate powder (autoclavable) 10L bottle No Price        
23-51-010-PC MEM, w/ Earle's salts, w/o L-glutamine, sodium bicarbonate powder (autoclavable) 50L bottle $49.92
1/EA
Add To Favorites      
23-90-009-PB MEM w/ Earle's salts, w/o L-glutamine, phenol red, sodium bicarbonate powder 10L bottle $37.04
1/EA
Add To Favorites      
23-90-009-PC MEM w/ Earle's salts, w/o L-glutamine, phenol red, sodium bicarbonate powder 50L bottle $127.38
1/EA
Add To Favorites      

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