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Medium 199 (Mod.)

By Corning® cellgro®
Cat.No. Description Pack Size Shipped Storage Intended Use
Medium 199 (Mod.), 1X
23-10-060-CV with Earle's salts & L-glutamine 6 x 500 mL A R I
Medium 199 (Mod.), Powder
23-50-050-PB with Earle's salts & L-glutamine
without sodium bicarbonate
1 x 10 L A R IVD
23-50-050-PC with Earle's salts & L-glutamine
without sodium bicarbonate
1 x 50 L A R IVD
23-90-050-PB with Earle's salts
without sodium bicarbonate, L-glutamine, & phenol red
1 x 10 L A R IVD
23-90-050-PC with Earle's salts
without sodium bicarbonate, L-glutamine, & phenol red
1 x 50 L A R IVD
23-50-051-PB with Hanks' salts & L-glutamine
without sodium bicarbonate
1 x 10 L A R IVD
23-50-051-PC with Hanks' salts & L-glutamine
without sodium bicarbonate
1 x 50 L A R IVD

F = -5 to -20°C
R = 2 to 8°C
A = 15 to 30°C
* = Glass
** = Flexible Media Bag
I = Sterile, IVD Product
NI = Sterile, Research Use Only, Not for IVD Use
IVD = In Vitro Diagnostic
Res = Research Use Only, Not for IVD Use

Medium 199 Formulation

Catalog No. 23-10-060
1X, Liquid
M199E
23-50-050
Powder
M199E
23-50-051
Powder
M199H
23-90-050
Powder
M199E
Inorganic Salts        
CaCl2 (anhydrous) 200.00mg/L 200.00mg/L 140.00mg/L 200.00mg/L
Fe(NO3)3•9H2O 0.72 0.72 0.72 0.72
KCl 400.00 400.00 400.00 400.00
KH2PO4 - - 60.00 -
MgSO4 (anhydrous) 97.70 97.70 97.70 97.70
NaCl 6800.00 6800.00 8000.00 6800.00
NaH2PO4•H2O 140.00 140.00 - 140.00
Na2HPO4 (anhydrous) - - 47.50 -
NaHCO3 2200.00 - - -
Amino acids
L-Alanine 25.00 25.00 25.00 25.00
L-Arginine•HCl 70.00 70.00 70.00 70.00
L-Aspartic acid 30.00 30.00 30.00 30.00
L-Cysteine•HCl•H2O 0.11 0.11 0.11 0.11
L-Cystine•2HCl 26.00 26.00 26.00 26.00
L-Glutamic acid 75.00 75.00 75.00 75.00
L-Glutamine 100.00 100.00 100.00 -
Glycine 50.00 50.00 50.00 50.00
L-Histidine•HCl•H2O 21.88 21.88 21.88 21.88
Hydroxy-L-proline 10.00 10.00 10.00 10.00
L-Isoleucine 20.00 20.00 20.00 20.00
L-Leucine 60.00 60.00 60.00 60.00
L-Lysine•HCl 70.00 70.00 70.00 70.00
L-Methionine 15.00 15.00 15.00 15.00
L-Phenylalanine 25.00 25.00 25.00 25.00
L-Proline 40.00 40.00 40.00 40.00
L-Serine 25.00 25.00 25.00 25.00
L-Threonine 30.00 30.00 30.00 30.00
L-Tryptophan 10.00 10.00 10.00 10.00
L-Tyrosine•2Na•2H2O 57.87 57.87 57.87 57.87
L-Valine 25.00 25.00 25.00 25.00
Vitamins
DL-alpha-Tocopherol•PO4•Na 0.01 0.01 0.01 0.01
Ascorbic acid 0.05 0.05 0.05 0.05
Biotin 0.01 0.01 0.01 0.01
Calciferol 0.10 0.10 0.10 0.10
D-Calcium pantothenate 0.01 0.01 0.01 0.01
Choline chloride 0.50 0.50 0.50 0.50
Folic acid 0.01 0.01 0.01 0.01
i-Inositol 0.05 0.05 0.05 0.05
Menadione•NaHSO3•3H2O 0.019 0.019 0.019 0.019
Nicotinic acid 0.025 0.025 0.025 0.025
Nicotinamide 0.025 0.025 0.025 0.025
Para-Aminobenzoic acid 0.05 0.05 0.05 0.05
Pyridoxine•HCl 0.05 0.05 0.05 0.05
Riboflavin 0.01 0.01 0.01 0.01
Thiamine•HCl 0.01 0.01 0.01 0.01
Vitamin A acetate 0.14 0.14 0.14 0.14
Other
Adenine sulfate 10.00 10.00 10.00 10.00
5-Adenylic acid•H2O 0.20 0.20 0.20 0.20
ATP, 2Na•3H2O 1.00 1.00 1.00 1.00
Cholesterol 0.20 0.20 0.20 0.20
2-Deoxy-D-ribose 0.50 0.50 0.50 0.50
D-Glucose 1000.00 1000.00 1000.00 1000.00
Glutathione (reduced) 0.05 0.05 0.05 0.05
Guanine•HCl 0.30 0.30 0.30 0.30
Hypoxanthine, Na 0.354 0.354 0.354 0.354
Phenol red, Na 10.00 10.00 10.00 -
D-Ribose 0.50 0.50 0.50 0.50
Sodium acetate 50.00 50.00 50.00 50.00
Thymine 0.30 0.30 0.30 0.30
Tween 80 20.00 20.00 20.00 20.00
Uracil 0.30 0.30 0.30 0.30
Xanthine, Na 0.34 0.34 0.34 0.34
Add
NaHCO3
Powder 7.5% Solution

-
-

2.20g/L
29.33mL/L

0.35g/L
4.70mL/L

2.20g/L
29.33mL/L
L-Glutamine
Powder
200 mM Solution

-
-

-
-

-
-

100.00mL/L
3.40mL/L
Specifications
pH (before buffer) N/A 4.5±0.5 4.5-6.0 4.5±0.5
pH (after buffer) 7.2±0.2 7.5±0.5 6.9±0.2 7.5±0.5
Osmolality (mOsm) 290±30 260-320 275-315 260-320
Grams of powder required to prepare 1L (1X Solution) N/A 9.50 10.63 9.39
                 
Cat.No. Name Pkg Size EA     PK    
23-10-060-CV Medium 199 (Mod.) w/Earle's salts, L-glutamine 6-pack/500mL bottles       $109.27
1/PK
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23-50-050-PB Medium 199 (Mod.) w/Earle's salts, L-glutamine, w/o sodium bicarbonate 10L (powder) $38.22
1/EA
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23-50-050-PC Medium 199 (Mod.) w/Earle's salts, L-glutamine, w/o sodium bicarbonate 50L (powder) $42.88
1/EA
Add To Favorites      
23-50-051-PB Medium 199 (Mod.) w/Hank's salts, L-glutamine, w/o sodium bicarbonate 10L (powder) DISCONTINUED        
23-50-051-PC Medium 199 (Mod.) w/Hank's salts, L-glutamine, w/o sodium bicarbonate 50L (powder) DISCONTINUED        
23-90-050-PB Medium 199 (Mod.) w/Earle's salts, w/o sodium bicarbonate, phenol red, L-glutamine 10L $72.52
1/EA
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23-90-050-PC Medium 199 (Mod.) w/Earle's salts, w/o sodium bicarbonate, phenol red, L-glutamine 50L $151.73
1/EA
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