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Ham's F-12 Medium

By Corning® cellgro®

Shipped at 15 to 30°C; store at 2 to 8°C.

Ham's F-12 Medium Formulation

Catalog No. 23-10-080 1X, Liquid
Ham's F-12
23-50-040 Powder
Ham's F-12
Inorganic Salts
CaCl2 (anhydrous) 33.30mg/L 33.30mg/L
CuSO4 (anhydrous) 0.0016 0.0016
FeSO4•7H2O 0.834 0.834
KCl 223.60 223.60
KH2PO4 - -
MgSO4 (anhydrous) 72.20 72.20
NaCl 7600.00 7600.00
Na2HPO4 (anhydrous) 142.00 142.00
NaHCO3 1176.00 -
ZnSO4•7H2O 0.863 0.863
Amino acids
L-Alanine 8.90 8.90
L-Arginine•HCl 211.00 211.00
L-Asparagine•H2O 15.00 15.00
L-Aspartic acid 13.30 13.30
L-Cysteine•HCl•H2O 35.12 35.12
L-Glutamic acid 14.70 14.70
L-Glutamine 146.20 146.20
Glycine 7.50 7.50
L-Histidine•HCl•H2O 20.96 20.96
L-Isoleucine 3.94 3.94
L-Leucine 13.10 13.10
L-Lysine•HCl 36.50 36.50
L-Methionine 4.48 4.48
L-Phenylalanine 4.96 4.96
L-Proline 34.50 34.50
L-Serine 10.50 10.50
L-Threonine 11.90 11.90
L-Tryptophan 2.04 2.04
L-Tyrosine•2Na•2H2O 7.84 7.84
L-Valine 11.70 11.70
Vitamins
Biotin 0.0073 0.0073
D-Calcium pantothenate 0.48 0.48
Choline chloride 13.96 13.96
Folic acid 1.30 1.30
i-Inositol 18.02 18.02
Nicotinamide 0.037 0.037
Pyridoxine•HCl 0.062 0.062
Riboflavin 0.038 0.038
Thiamine•HCl 0.34 0.34
Vitamin B12 1.36 1.36
Other
D-Glucose 1802.00 1802.00
Hypoxanthine, Na 4.77 4.77
Lipoic acid 0.21 0.21
Methyl lineoleate 0.088 0.088
Phenol red, Na 1.20 1.20
Putrescine•2HCl 0.16 0.16
Sodium pyruvate 110.00 110.00
Thymidine 0.73 0.73
Add
NaHCO3
Powder
7.5% Solution

-
-

1.176g/L
15.70mL/L
Specifications
pH (before buffer) N/A 6.5±0.5
pH (after buffer) 7.2±0.2 7.5±0.5
Osmolality (mOsm) 290±30 290±30
Grams of powder required to prepare 1L (1X Solution) N/A 10.64
                 
Cat.No. Name Pkg Size EA     PK    
23-10-080-CM Ham's F-12 Medium w/ L-glutamine 6-pack/1L bottles       $177.24
1/PK
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23-10-080-CV Ham's F-12 Medium w/ L-glutamine 6-pack/500mL bottles       $105.73
1/PK
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23-50-040-PB Ham's F-12 Medium w/ L-glutamine 10L (powder) $35.36
1/EA
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23-50-040-PC Ham's F-12 Medium w/ L-glutamine 50L (powder) $57.95
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