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Mastercycler® ep Thermal Cyclers

By Eppendorf
Mastercycler® ep Thermal Cyclers

Speed, precision, more user-friendliness and absolute reliability united within a flexible concept characterize the Mastercycler® ep system. Premium features include thermoblocks with extremely fast heating and cooling speeds and unique heated lid technology, which avoids the preheating of samples and minimizes unspecific annealing.

The gradient option of each Mastercycler® ep system features unique SteadySlope® technology to ensure that the heating and cooling ramp rates are identical in both gradient and normal operation—that means you can expect identical temperature control characteristics in both optimization and routine experiments, as well as a reliable transfer of the optimization results to the routine application. Programming with the Control Panel and PC software is easy and intuitive.

For integration into fully automated environments Mastercycler® ep models are available with a optional motorized lid. And for unsurpassed speed, choose the Mastercycler® ep gradient S—it even enables a device-driven Hot Start, also known as Impulse PCR.


  • Extremely fast heating and cooling rates
  • Gradient blocks with SteadySlope™ technology
  • Intuitive graphic programming
  • Can be used as a stand-alone device, a "Mini-Satellite" system, or a PC-controlled network
  • Heated lid with integrated ESP technology (Electronic Sample Protection)
  • Optional motorized lid for integration into fully automated environments
  • Licensed for PCR
  • Two-year warranty
Model: Mastercycler® ep gradient Mastercycler® ep gradient S Mastercycler® ep 384
Sample Capacity: 96 x 0.2mL PCR tubes or 1 PCR plate 8 x 12
(unskirted, semi-skirted, skirted—according to SBS standard)
1 PCR plate 384
Block Temperature Control Range: 4°C to 99°C
Temperature Control Mode: Block control, (simulated) tube control;
both also available in gradient operation
Block Heating Technology: Peltier elements, Triple Circuit
Gradient Range: 1°C to 20°C 1°C to 24°C 1°C to 20°C
Gradient / Lid Temperature Range: 30°C to 99°C / 37°C to 110°C
Lid Descent and Closing Pressure: ESP technology, max. of 20kg / PCR plate
Block Homogeneity
20°C to 72°C:
≤ ±0.3°C
≤ ±0.4°C
Control Accuracy: ±0.2°C
Heating Rate:
Cooling Rate:
ca. 4°C/s
ca. 3°C/s
ca. 6°C/s
ca. 4.5°C/s
ca. 4°C/s
ca. 3°C/s
Interfaces: 1 x Centronics, 1 x RS-232, Control panel, one each of CAN_in/ CAN_out
Dimensions (W x D x H):
10.4 x 16.4 x 12.2" (26 x 41 x 30.5cm)
37.5 lb (17kg)
Power Supply / Consumption: 120V, 50–60Hz / 800W

Mastercycler® ep Thermal Cyclers

Cat.No. Name Electrical EA    
38-950010029 Mastercycler® ep Gradient Thermal Cycler with motorized lid 120V DISCONTINUED  
38-950010045 Mastercycler® ep Gradient S Thermal Cycler with motorized lid 120V DISCONTINUED  
38-950010061 Mastercycler® ep 384 Thermal Cycler with motorized lid 120V DISCONTINUED  
38-950011009 Control Panel for Mastercycler® ep systems (includes connection cable) 120V DISCONTINUED  

Connection Cables

Cat.No. Name EA    
38-950014008 CAN-Bus Connection Cable, 50cm $46.13
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38-950014016 CAN-Bus Connection Cable, 150cm $56.38
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