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X-Gal

By Amresco
X-Gal

Storage Condition: FROZEN

CAS Number: 7240-90-6

Molecular Weight: 408.64

Molecular Formula: C14H15BrClNO6

Used in conjunction with IPTG to identify bacterial colonies that contain recombinant plasmids by blue/white selection. X-Gal forms an intense blue precipitate in the presence of β-Galactosidase. Insoluble. Reaction Condition: Stocks: X-Gal 20 mg/mL in DMF; IPTG 20 mg/mL in water. Mix 4:1 (X-Gal/IPTG) for spreading onto agar plates prior to plating transformed bacteria.

Blank (A620; 0.1%, PO4, pH 7.3) 0.01
Identification (P/F) Pass
Purity (Enzymatic) 99.0%
Solubility (2%, DMF) (P/F) Pass
Specific Rotation (0.1%, 50% DMF) -64 - -56°

           
Cat.No. Size Grade EA    
1328-0428-100MG 100mg Ultra Pure DISCONTINUED  
1328-0428-1G 1g Ultra Pure DISCONTINUED  

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