- High sensitivity
- Complete kits of high quality reagents
- All reagents are nuclease-free
Amresco's TotalBLOT™ Southern Kits are complete kits for electrophoresis and transfer of DNA samples onto a nylon membrane using high salt buffer. Each kit provides all of the necessary reagents for the detection and analysis of nucleic acids. All the reagents are tested for nuclease contamination to ensure quality results.
Kits include: Agarose 1™, 10g; TBE (Tris-Borate-EDTA) Buffer, 10X, 1L Pack Depurination Solution, 1L; Denaturing Solution, 1L; Neutralizing Solution, 1L; SSC Buffer, 20 x 4L.
Kits are available with or without positively charged nylon membranes. TotalBLOT+™ Nylon Blotting Membranes are also available separately, and provide high binding capacity and are resistant to tearing and cracking.
|Agarose Nuclease (P/F)||None|
|Denaturing Solution Nuclease (P/F)||None|
|Denaturing Solution pH||11.5|
|Depurination Solution pH||2.0|
|Neutralizing Solution pH||6.9 - 7.1|
|SSC Buffer pH||6.85 - 7.15|
|TBE Buffer pH||8.1 - 8.5|