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Rapid Transfer Buffer, 10X

By Amresco
Rapid Transfer Buffer, 10X

Storage Condition: ROOM TEMPERATURE

Risk Statements: 36/37/38-7

Safety Statements: 36/37/39-15

Hazard Code: irritant, combustible

  • Fast, efficient transfer in 10 - 20 minutes
  • Compatible with both wet & semi-dry transfer equipment
  • Methanol-free, non-hazardous formulation
  • Transfers to PVDF and nitrocellulose from multiple types of gels

Amresco's Rapid Transfer Buffer is a simple, single component system for quick and efficient transfer of proteins from SDS-PAGE gels to membranes for Western blotting applications. Transfer is completed in 10 to 20 minutes using a standard semi-dry or wet transfer apparatus, respectively. Rapid Transfer Buffer is a methanol-free, non-hazardous formulation that is compatible with PVDF and nitrocellulose membranes. It is used for transfer of proteins from standard Laemmli gels, commercial pre-cast gels, and Amresco’s NEXT GEL™. The transfer efficiency is equivalent to that observed when using a Tris-Glycine-Methanol transfer buffer.

Dilute stock solution to prepare 1X buffer for wet or semi-dry protein transfer.

Glycine Titration (M) 1.85 - 1.99
pH (1X, Water) @ 25°C 8.1 - 8.4
Protease (P/F) None
Tris Titration (M) 0.2 - 0.3

Cat.No. Size Grade EA    
1328-N789-100ML-SAMPLE 100mL Sample Biotechnology DISCONTINUED  
1328-N789-1L 1L Biotechnology DISCONTINUED  
1328-N789-4L 4L Biotechnology DISCONTINUED  

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