Advanced Search

Cytoplasmic / Nuclear Protein Enrichment Kit

By Amresco
Cytoplasmic / Nuclear Protein Enrichment Kit

Storage Condition: COLD

This kit isolates proteins from both the cytoplasmic and nuclear fractions of cultured cells. The cytoplasm and the nucleus are separated from one another by simple centrifugation steps following lysis in a non-ionic detergent. The entire procedure can be performed in less than 1.5 hours. Kit includes Fractionation Buffer 1 (1mL), Fractionation Buffer 2 (1.5mL), Fractionation Buffer 3 (30mL), and Nonidet® P-40 Substitute (0.1mL). Contains sufficient material for 20 extractions of 1 x 106 cells.

Resuspend cell pellet in a cytoplasmic lysis buffer for 15 minutes. Add detergent and pellet the nuclei. Transfer the supernatant containing the cytoplasmic fraction and further isolate the proteins with cytoplasmic extraction buffer. After the nuclei have been washed, the pellet is resuspended in nuclei lysis buffer for 15 minutes. The nuclei debris is pelleted and the supernatant contains the nuclei protein fraction.

  • Generate distinct nuclear and cytoplasmic fractions from cultured cells in 90 minutes
  • For adherent or non-adherent cultured cells
  • Compatible with downstream Western blotting applications
Cat.No. EA   Certified

The following items have been added to your cart:

Choose a favorite list for this item:

Catalog Number Description Price

Request Literature

Please fill out the form below if you want to request literature from Krackeler Scientific.


Quick Order

* Required

New Year Price Updates

We are currently working diligently to update our website pricing information for the New Year. If you place an order, you will be acknowledged with any corrected pricing. If you'd like the most current information sooner, please don't hesitate to drop us an email or give us a call and we'd be happy to assist. Thank you for your patience while we are updating.


Play Video

To Request a Quote

  1. Search or Browse for items and add to them to your Shopping Cart.
  2. Click the "Request Quote" button at the bottom of the Shopping Cart page.
  3. Fill out required fields.
  4. Optionally you can convert to standard checkout mode by choosing a payment type.
  5. Click "Request Quote" at the bottom of the page.

You will be contacted with a quote.

To Order From a Quote

  1. Register and login to the website.
  2. Receive a quote from your sales representative or customer service.
  3. Have your copy of the quote in hand.
  4. Visit our quote module to search for your quote.
Back to Top