Attachment Factors

BD Cell-Tak™ Cell and Tissue Adhesive is a formulation of polyphenolic proteins extracted from Mytilus edulis (marine mussel). These proteins are the key components of the glue secreted by the mussel to anchor itself to solid substrates in its marine environment. BD Cell-Tak Cell and Tissue Adhesive is used to attach cells or tissue sections to many types of surfaces, including plastic, glass, metal, FEP Polymer, and biological materials. BD Cell-Tak Cell and Tissue Adhesive is biocompatible and demonstrates no species specificity. It can simplify the manipulation of biological samples for a number of in vitro techniques, including in situ hybridization, immunoassays, microinjection, immunohistochemistry, and establishing primary cells in culture.

Poly-D-Lysine (PDL) is a synthetic molecule used as a coating to enhance cell attachment to plastic and glass surfaces. It has been used to culture a wide variety of cell types, particularly neurons, glial cells and transfected cells. PDL is commonly used as a culture substrate to promote adhesion, growth, and differentation for a variety of neuronal and transfected cell lines.

BD Matrigel™

BD Matrigel Basement Membrane Matrix is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix proteins. Its major component is laminin, followed by collagen IV, heparan sulfate proteoglycans, entactin/nidogen. BD Matrigel Basement Membrane Matrix also contains TGF-beta, epidermal growth factor, insulin-like growth factor, fibroblast growth factor, tissue plasminogen activator, and other growth factors which occur naturally in the EHS tumor. BD Matrigel Basement Membrane Matrix is effective for the attachment and differentiation of both normal and transformed anchorage dependent epithelioid and other cell types.

BD™ Collagen

Collagen I is found in most tissues and organs, but is most plentiful in dermis, tendon, and bone. It can be used as a thin layer on tissue-culture surfaces to enhance cell attachment and proliferation, or as a gel to promote expression of cell-specific morphology and function. Type 1 collagen is commonly used to culture endothelial cells, hepatocytes, muscle cells, and a variety of other cell types.

Type II Collagen is the pricipal collagenous component of cartilage, intervertebral disc and vitreous humour. It is also found in other tissues during development. Its main function is to provide tensile strength and give cartilage the ability to resist shearing forces. Type II Collagen supports chondrocyte adhesion and may influence the differentiated phenotype of these cells.  It can also be used as an in vivo model in rats and mice for arthritis studies (injection of Bovine Collagen II induces arthritis). 

Type III Collagen is found co-localized with Type I Collagen in tissues, blood vessels and skin. Type III Collagen is a homotrimeric procollagen comprised of three identical pro a, (III) chains.  Collagen III is important for the development of skin and the cardiovascular system and for maintaining normal physiological functions in adult life.  Collagen III has been used for studies of Collagen I fibrillogenesis in normal cardiovascular development.  Many of the same properties and functions of animal derived collagens can be duplicated using recombinant human collagens.  FIBROGEN Recombinant Human Collagens Type I and III from BD Biosciences are highly purified, reliable and consistent. They provide alternatives to animal derived collagens for biomedical applications in which an enhanced safety profile, and greater reproducibility and quality are critical.

Collagen VI is a large, multidomain extracellular matrix protein. Its heterotrimeric chains assemble into a microfibrillar network via tetramerization and end-to-end association1. It‘s pattern of distribution and its unique structure and expression compared with other ECM molecules indicate that Collagen VI may fulfill specialized tasks in tissue organization and cell functioning. 

Collagen V is found in whole placenta, amnion, chorion, and cornea. It can be used as a thin coating on tissue-culture surfaces to study collagen V effects on cell behavior. Collagen V has been shown to inhibit endothelial cell proliferation selectively. 

Collagen VI is a large, multidomain extracellular matrix protein. Its heterotrimeric chains assemble into a microfibrillar network via tetramerization and end-to-end association1. It‘s pattern of distribution and its unique structure and expression compared with other ECM molecules indicate that Collagen VI may fulfill specialized tasks in tissue organization and cell functioning. 

Cell Detachment/Recovery Reagents

BD™ Cell Recovery Solution allows for the recovery of cells cultured on BD Matrigel™ Basement Membrane Matrix for subsequent biochemical analyses. BD™ Cell Recovery Solution depolymerizes BD Matrigel Matrix gels without enzymatic digests and lengthy incubation periods at high temperatures. Cells are released without damage thereby avoiding biochemical changes during incubation and digestion of extracellular portions of cell-surface receptors and adhesion molecules.

Glycoproteins

Fibronectin (FN) is found in interstitial matrix and plasma. Cell adhesion to fibronectin is mediated by the central cell-binding domain of FN through RGD (Arg-Gly-Asp) sequence. Fibronectin's principal function appears to be in cellular migration during wound healing and development. It can be used as a thin coating on tissue-culture surfaces to promote attachment, spreading, and proliferation of a variety of cell types. Fibronectin can also be used as an additive to serum-free culture medium. 

Laminin, a major component of basement membranes, has numerous biological activities including promotion of cell adhesion, migration, growth, and differentiation, including neurite outgrowth. It can be used as a thin coating on tissue-culture surfaces or as a soluble additive to culture medium. Laminin has been shown in culture to stimulate neurite outgrowth, promote cell attachment, chemotaxis, and cell differentation. 

Vitronectin, also known as S-protein, serum spreading factor and epibolin, is a mixture of two monomeric glycoproteins (65 and 75 kD) present in blood and the extracellular matrix (ECM) of many tissues. Vitronectin and fibronectin are the two major adhesive proteins in plasma and serum.

Osteopontin is found in a number of tissues, including bone, placenta, distal tubules of the kidney, and the central nervous system. Osteopontin is expressed in early bone development, at high levels at sites of bone remodeling, and has been implicated in the process of osteogenesis. Osteopontin is chemotactic for macrophages, smooth muscle cells, endothelial cells, and glial cells. In culture, osteopontin is used as an adhesive substrate for tumor cell lines and osteoclasts.

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