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All Categories >> Cell Culture Media >> Cell Culture Reagents

McCoy's 5A (Iwakata & Grace Modification)

Shipped at 15 to 30°C; store at 2 to 8°C.

McCoy's 5A Medium Formulation

Catalog No. 23-10-050
1X, Liquid
McCoy's 5A
23-10-051
1X, Liquid
McCoy's 5A
23-50-025
Powder
McCoy's 5A
Inorganic Salts
CaCl2 (anhydrous) 100.00mg/L 100.00mg/L 100.00mg/L
KCl 400.00 400.00 400.00
MgSO4 (anhydrous) 97.70 97.70 97.70
NaCl 6460.00 5960.00 6460.00
NaH2PO4•H2O 580.00 580.00 580.00
NaHCO3 2200.00 2200.00 -
Amino acids
L-Alanine 13.36 13.36 13.36
L-Arginine•HCl 42.14 42.14 42.14
L-Asparagine•H2O 45.03 45.03 45.03
L-Aspartic acid 19.97 19.97 19.97
L-Cysteine•HCl•H2O 35.14 35.14 35.14
L-Glutamic acid 22.10 22.10 22.10
L-Glutamine 219.20 219.20 219.20
L-Glycine 7.50 7.50 7.50
L-Histidine•HCl•H2O 20.96 20.96 20.96
Hydroxy-L-proline 19.70 19.70 19.70
L-Isoleucine 39.36 39.36 39.36
L-Leucine 39.36 39.36 39.36
L-Lysine•HCl 36.54 36.54 36.54
L-Methionine 14.92 14.92 14.92
L-Phenylalanine 16.52 16.52 16.52
L-Proline 17.30 17.30 17.30
L-Serine 26.30 26.30 26.30
L-Threonine 17.90 17.90 17.90
L-Tryptophan 3.10 3.10 3.10
L-Tyrosine•2Na•2H2O 26.12 26.12 26.12
L-Valine 17.60 17.60 17.60
Vitamins
Ascorbic acid 0.50 0.50 0.50
Biotin 0.20 0.20 0.20
D-Calcium pantothenate 0.20 0.20 0.20
Choline chloride 5.00 5.00 5.00
Folic acid 10.00 10.00 10.00
i-Inositol 36.00 36.00 36.00
Nicotinamide 0.50 0.50 0.50
Nicotinic acid 0.50 0.50 0.50
Para-Aminobenzoic acid 1.00 1.00 1.00
Pyridoxine•HCl 1.00 1.00 1.00
Riboflavin 0.20 0.20 0.20
Thiamine•HCl 0.20 0.20 0.20
Vitamin B12 2.00 2.00 2.00
Other
Bacto-peptone 600.00 600.00 600.00
D-Glucose 3000.00 3000.00 3000.00
Glutathione (reduced) 0.50 0.50 0.50
HEPES - 5958.00 -
Phenol red, Na 10.00 10.00 10.00
Add
NaHCO3
Powder
7.5% Solution

-
-

-
-

2.2g/L0
29.33mL/L
Specifications
pH (before buffer) N/A N/A 5.0±0.5
pH (after buffer) 7.2±0.2 7.2±0.2
@ 20-25°C
7.0±0.5
Osmolality (mOsm) 290±30 305±30 290±30
Grams of powder required to prepare 1L (1X Solution) N/A N/A 12.01

 

Cat.No.NamePkg SizePK
23-10-051-CIMcCoy's 5A (Iwakata & Grace Modification), 1X w/L-glutamine, 25mM HEPES6-pack/100mL bottles$100.46
PK of 1
23-10-050-CVMcCoy's 5A (Iwakata & Grace Modification), 1X w/L-glutamine6-pack/500mL bottles$121.07
PK of 1





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