An in vitro culture system for rapid proliferation of SMC from human and rat aorta. Includes SMC P-STIM™ Proliferation Medium.

• Rapid monolayer formation with absence of heavy chain myosin and 150 kD h-caldesmon expression
• 90% cell attachment within one-two hours
• 90% of attached cells spread within two-four hours
• Cell population doubles three to six times within five to six days
• Reduced feedings save labor time and media

Complete protocol provides:

• Capability for immediate start-up

Applications:

• SMC proliferation
• Study of molecular mechanisms of phenotypic transitions
• Screening of antiproliferative compounds