An in vitro system optimized for the differentiation of human and rat SMC. Includes specially formulated BD BioCoat™ Growth Factor Reduced (GFR) Matrigel™ Matrix Cellware and serum-free BD Smooth Muscle Differentation Medium.

• Three-dimensional matrix promotes differentiated (contractile) SMC phenotype, low proliferative index, nodular morphology and ultrastructure dominated by microtubules and microfilaments
• Expression of cytoskeletal markers, α-smooth muscle actin, heavy chain myosin, and 150 kD h-caldesmon
• Carry out studies of SMC specific function two weeks earlier than Collagen I
• Reduced levels of growth factors for cytokine studies
• Serum-free environment maximizes control over experimental conditions

Complete protocol provides:

• Standardized procedures
• Capability for immediate start-up

Applications:

BD Matrigel™ Matrix has been used to culture SMC for studies of:

• SMC differentiation
• Modulation of SMC function
• Molecular mechanisms of phenotypic transitions
• Effects of various compounds on SMC behavior
• Effects of ECM proteins on vascular SMC phenotype