An in vitro system for the study of cell invasion through basement membrane. Consists of BD Falcon™ Cell Culture Inserts containing an 8 µm pore-size PET membrane coated with a uniform layer of BD Matrigel™ Basement Membrane Matrix.

• Discriminating, reproducible, ready-to-use
• Invasivity of HT-1080 cells is at least fivefold greater than 3T3 cells
• Uniform layer of BD Matrigel Matrix occluding the 8 µm pores of PET microporous membrane
• An authentic model of in vivo basement membrane
• Barrier to noninvasive cells
• Proprietary coating process
• Only invasive cells digest the matrix and move through the insert membrane

Complete protocol provides:

• Standardized procedures
• Capability for immediate start-up
• Instructions for quantitating percent invasion

Applications:

BD BioCoat™ Matrigel Invasion Chambers enable studies of:

• Metastatic potential of tumor cells
• Expression of matrix metalloproteinase on surface of invasive tumor cells
• Inhibition of metastasis by ECM components or antineoplastic drugs (i.e., taxol)
• Altered expression of cell surface proteins in metastatic cells
• Invasion of normal cells, such as embryonic stem cells cytotrophoblasts and fibroblasts
• Effects of doxycycline on invasion and metalloproteinase activity of breast carcinoma cells
• Metastatic potential of a newly isolated bladder cancer cell line
• Suppression of lung metastasis of mouse melanoma by gene transfection