Poly-D-Lysine (PDL) is a synthetic compound that enhances cell adhesion and protein absorption by altering surface charges on the culture substrate. In addition to promoting cell adhesion, poly-lysine surface treatments support neurite outgrowth and improve the survival of many central nervous system (CNS) primary cells in culture. Because PDL is a synthetic molecule, it does not stimulate biological activity in the cells cultured on them, and does not introduce impurities carried by natural polymers.

BD BioCoat™ Poly-Lysine Cellware offers tissue-culture vessels with a uniform application of poly-lysine.

Applications include:

• Attachment and spreading of a variety of cell types
• Cell differentiation and neurite outgrowth
• Attachment of fastidious transfected cell lines
• Support survival of primary neurons in culture
• Serum-free or reduced serum culture